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In this study, the unpretreated sugar cane bagasse wa milled to a particle size of 0.5 – 1 mm and be used as material for bioconversion into f ermentable sugars by using an enzyme cocktail acted synergistically. Experimental planni ng was used to optimize the enzyme conversion through assessment and analysis of indiv idual parameter. As the result, the optimal condition for enzymatic conversion of sugar cane ba gasse into reducing sugar product are at pH = 5, 40 C, and 48 h incubation in rate of enzyme (Cell/Xyl, AltFAE, XpoAE) and substrate (bagasse meal) is 3.1. From the above application, the mathematical model is found to des cribe equation of the bioconversion of bagasse into reduc ing sugars : y = 206.946 + 29.954x 1 + 5.501x2 + 7.323x3 + 2.288x2x3 – 7.011 2 1 x ; and using flexible algorithm of nonlinear planning to identify optimal conditions of enzyme mixture of conversion into reducing sugars that the reaction r eached y max = 251.86 mg per gram bagasse with x 1 = 1.215, x2 = 1.215, x3 = 1.215 or Cell/Xyl = 1 ml (100U), AltFAE = 0.5 ml (7.56U), XpoAE = 0.4 ml (10.8U) on the test range. Experimental verification has the same result in constant conditons and reached total redu cing sugars of 260.2 mg per gram substrate.

vietnam journal of science and technology/science and technology

USING EXPERIMENTAL PLANNING TO OPTIMIZE THE HYDROLYSIS OF SUGAR CANE BAGASSE INTO FERMENTABLE SUGARS FOR BIOETHANOL PRODUCTION BY FUNGAL ENZYME MIXTURE