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STUDY ON EFFECT OF IMMUNE STIMULATION OF γ-RAY IRRADIATED β-GLUCAN ON TILAPIA Nguyen Ngoc Duy, Dang Van Phu, Nguyen Quoc Hien Research and Development Center for Radiation Techn ology, Vietnam Atomic Energy Institute 202A, Str. 11, Linh xuan Ward, Thu duc District, Ho Chi Minh City Email: ngocduy158@yahoo.com Low molecular weight β-glucan (LMWβG) and oligoβ-glucan solution were prepared by the hydrothermal steaming combination with γ-irradiation method. The efficiency of the degradation process was demonstrated by gel permeat ion chromatography (GPC) analysis of the average molecular weight (Mw) of β-glucan. Results showed that the Mw decreased with increasing steaming time, concentration of H 2O2 and doses. For LMWβG, Mw reduced from 296,600 Da to 44,400 Da when concentration of H 2O2 raises from 0 to 10 % and for oligo βglucan Mw reduced from 56,700 to 7,100 Da at 16 kGy . Tilapia fish was fed with LMWβ and oligoβ-glucan of 100 mg/kg feed for 45 days and then wa s challenged with Streptococcus agalactiae bacterial to investigate immune stimulation . The results indicated that oligo β-glucan has higher immune stimulation effect compared to LM WβG. The effect of oligo β-glucan with various concentrations of 50, 100 and 150 mg/kg fee d was investigated. Results showed that the highest survival rate was attained for oligo β-glucan of 150 mg/kg as feed additive.

NGHIÊN CỨU HIỆU ỨNG KÍCH KHÁNG BỆNH CỦA β-GLUCAN CẮT MẠCH BẰNG PHƯƠNG PHÁP CHIẾU XẠ ĐỐI VỚI CÁ RÔ PHI