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Serum testosterone immunoassay interference may cause falsely high results. We report a case of 20-year-old female with PCOS whose initial serum testosterone levels were more than 10 nM. Further imaging revealed a left adrenal adenoma. During an attempt at bilateral adrenal-ovarian venous sampling at another institution, her peripheral serum total testosterone levels on a different assay platform were surprisingly normal. Subsequently, simultaneous samples performed on three different assay platforms confirmed the presence of assay interference from the originating institution.

Falsely Elevated Serum Testosterone Levels in a Young Adult Female with Polycystic Ovarian Syndrome (PCOS) and Left Adrenal Adenoma