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Analytical methods using capillary tubes have attracted a great deal of interest since the last century [1-3]. Well-known methods include capillary electrophoresis, capillary electrochromatography, micellar electrokinetic capillary chromatography, and highperformance liquid capillary chromatography using packed and monolithic columns. However, only a few new concepts concerning capillary chromatography have been reported in the last decade [4,5]. Related research has been discussed in detail in our previous papers [6,7]. We have developed a capillary chromatography system using an open capillary tube and a water-hydrophilic/hydrophobic organic mixture carrier solution [6-11]. This system works under laminar flow conditions and does not require any packed reagents in the capillary tube or application of high voltage to the ends of the tube. It is called a tube radial distribution chromatography (TRDC) system. In the TRDC system [6,7], 1) various types of capillary tube are available, such as fused-silica, polyethylene, and poly (tetrafluoroethylene), 2) a ternary mixed solution, i.e. , waterhydrophilic/hydrophobic organic mixture carrier solution (homogeneous) must be delivered into the capillary tube under laminar flow conditions, 3) the elution times of analytes, hydrophobic and hydrophilic, are easily reversed by changing the component ratios of the carrier solvents, i.e. , by using an organic solvent-rich and a water-rich carrier solution, and 4) the first peak appears with almost the average linear velocity and the other peaks are eluted with lower velocities than the average linear velocity under laminar flow conditions in both organic solvent-rich and water-rich carrier solutions. Separation performance in the TRDC system is explained Original

chromatography

Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions