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The validity testing of the detection Vibrio parahaemolyticus AHPND strain in vaname shrimp (Litopenaeus vannamei) with the real time PCR method was carried out through stages which included specificity, sensitivity, repitability, reproducibility, and sampling field tests on vaname shrimp ponds in Indonesia. Analytical performance test showed that real time PCR method of the primer VpPirA specific in to detected Vibrio parahaemolyticus AHPD strain, and had a sensitivity of up to 10 copies µL -1 .  The cut off value qPCR method of the primer VpPirA is at Ct 39. The real time PCR method of the primer  VpPirA also has good repitability and reproducibility values in detecting Vibrio parahaemolyticus AHPND strain. All analytical performance testing of the qPCR method meets the acceptance requirements and can be used as a test method to detect Vibrio parahaemolyticus AHPND strain. Field test results for all samples originating from vaname shrimp ponds were negative.

DETEKSI Vibrio parahaemolyticus MENGGUNAKAN MARKA GEN PIR A PADA UDANG VANAME (Litopenaeus vannamei) DENGAN REAL TIME PCR