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Streptococcus mutans are bacteria that have important role in caries development. When caries lesion is developing, it may invade to deeper tissues such as dental pulp and initiate cellular defenses such as neutrophil. Neutrophil is type of leukocytes which are important in phagocytosis process. If neutrophil fails in phagocytosis, neutrophil will be lysis and cause viability of neutrophil unprotected. Temulawak rhizome extract is predicted to increase viability of neutrophil because it contains curcuminoids, volatile oil, saponin, flavonoid, and tannin. This research aimed of this research was to determine the viability of neutrophil exposed by S.mutans and incubated in temulawak rhizomes extract 2.5%, 5%, 7.5%,10%,and 20%.Twenty foursamples were divided into 6 groups,consisting of negative control, temulawak rhizome extract 2.5%, 5%, 7.5%, 10%, and 20%. The viability of neutrophil was observed under an inverted microscope using trypan blue staining. This study concluded that temulawak rhizome extract 2.5%, 5%, 7.5%, and 10% could increase viability of neutrophil exposed by S.mutans. Temulawak rhizome extract 5% has the effective concentration to increase viability of neutrophil.

journal of dentomaxillofacial science

Viabilitas neutrofil yangdiinkubasi dalamekstrakrimpangtemulawak(Curcuma xanthorrhiza ) dan dipapar dengan Streptococcus mutans (Viability of neutrophil incubated in temulawak rhizome extract (Curcuma xanthorrhiza) and exposed by Streptococcus mutans)