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We report  Acanthosyris annonagustata  (Santalaceae) for the flora of Peru, from an Amazon rainforest site in Madre de Dios Department. We describe the species using leaf and fruit morphological traits from field observations and botanical vouchers, and we provide demographic and phenological observations. This is the second verified species of  Acanthosyris  reported from the Peruvian Amazon and the third verified species for Peru. A key for the three species reported in Peru is provided.

Acanthosyris annonagustata C.Ulloa & P.Jørg. (Santalaceae), newly recorded for the flora of Peru