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Astyanax bifasciatus  Garavelo &amp; Sampaio, 2010 was originally described as endemic to the Iguaçu river basin. Between March 2017 and January 2018, specimens of  A. bifasciatus  were sampled during expeditions to headwater streams of the Piquiri river basin, upper Paraná river basin. The identification was confirmed both by morphological and molecular analyses, representing, therefore, the first record of the species outside of the basin of the Iguaçu River. In addition, the lack of structuring in the haplotype network confirms that the representatives of  A. bifasciatus  from both basins appear to comprise a single population.

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First record of  Astyanax bifasciatus  Garavello &amp; Sampaio, 2010 (Teleostei, Ostariophysi, Characidae) in the Piquiri river basin, upper Paraná river basin