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Tetraspora gelatinosa  is rare and has been recorded only in 3 Brazilian states since the 2000s. The flora of the state of Goiás is incipiently known, but there is no record of  Tetraspora  thus far. We record the occurrence of  T. gelatinosa  in Goiás and characterize this species’ morphology and ecological preferences. Specimens were found in the Samambaia Reservoir, Goiânia, Goiás. Physical and chemical characteristics of the water were measured. Where  T. gelatinosa  was found, the water was shallow and characterized as ultraoligotrophic. These conditions agree with those reported for other environments in Brazil.

First record of Tetraspora gelatinosa Link ex Desvaux (Tetrasporales, Chlorophyceae) in the state of Goiás, Central-Western Brazil