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We present a checklist of ferns and lycophytes from Parque Estadual Mata das Flores, a state park located in Castelo, Espírito Santo, Brazil. The park has an area of 800 ha, and its main vegetation is composed of dry forest (at 150–500 m elev.), and a small portion of wet forest (500–700 m), within the Brazilian Atlantic Rainforest biome. We found 83 taxa, including 75 species, 3 varieties, 4 putative hybrids, and 1 cultivar. Among these, 17 are newly recorded from the Espírito Santo. Twenty-five taxa are endemic to the Atlantic Forest biome, and 5 are endemic to southeastern Brazil, and 2 are narrow endemics to the states of Rio de Janeiro and Espírito Santo. Anemia blechnoides is the only vulnerable species found, according to the national red list. We found 4 naturalized species. Among the hybrids, 3 are reported for the first time in literature ( Blechnum asplenioides  ×  B. glandulosum ,  Campyloneurum decurrens  ×  C. repens , and  Doryopteris collina  ×  D. lorentzii ) and 1 for the first time in Brazil ( B. glandulosum  ×  B. polypodioides ).

Checklist of ferns and lycophytes from Parque Estadual Mata das Flores, Castelo, Espírito Santo, Brazil