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This note demonstrates the use of a DNA barcoding methodology in confirming new occurrence records of  Pellona castelnaeana  and  Pellona flavipinnis  in the Branco River sub-basin. The DNA barcode result was verified by identification based on morphological characters of both species. Thus, these records increase the species’ ranges by more than 600 km in the Amazon and show evidence of high genetic variability in  P. flavipinnis.

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Using DNA barcodes to confirm new records of Amazon longfin herrings  Pellona castelnaeana  Valenciennes, 1847 and  Pellona flavipinnis  (Valenciennes, 1837) (Clupeiformes: Pristigasteridae) in the Branco River sub-basin