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We report the occurrence of the Amazonian hylid  Boana alfaroi  outside of Ecuador for the first  time. The locality (Assis Brasil, state of Acre) lies in northern Brazil, approximately 1,280 km southeast from the easternmost records in Ecuador. Additionally, we describe the vocalization of a male of  B. alfaroi  from the Brazilian population, including a second type of note besides the advertisement note.  Boana alfaroi  may occur across Amazonian lowland forests of Ecuador, northern Peru, and the Brazilian states of Acre and Amazonas.

First record of Boana alfaroi (Caminer & Ron, 2014) (Anura: Hylidae) in Brazil