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Scentless chamomile ( Tripleurospermum inodorum  =  M. inodora ) is a plant belonging to Anthemideae tribe of Asteraceae family, with phenotype similar to the common chamomile, a plant used in human consumption in the form of herbal tea infusion. In order to be able to understand possible health-promoting properties and adverse effects of the scentless chamomile's consumption, it is of essence to examine its chemical composition. The aim of the study was to perform phenolic profiling using high-performance liquid chromatography–tandem mass spectroscopy (HPLC–MS/MS), in comparison to the common chamomile. In the investigated extracts, qualitative and quantitative analyses enabled the identification of 66 compounds based on their retention times, mass (MS/MS) spectra, and analysis of their characteristic fragmentation patterns in MS/MS Product Ion Scan experiments. A new HPLC–MS/MS method for quantitation of common plant metabolites was hereby developed, enabling quantitation of 47 compounds. All examined  M. inodora  samples have relatively high combined phenolic and flavonoid contents (25.2–51.9 mg/g). Apigenin, apigenin-7- O -glucoside, luteolin, luteolin-7- O -glucoside, quinic acid, and 5- O -caffeoyl quinic acid were the compounds with highest concentration in both inodorous and common chamomile. The results obtained hereby represent the first and most detailed chemical profile of scentless chamomile so far.

HPLC–MS/MS profiling of wild-growing scentless chamomile