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The surplus of waste glycerol, by-product of the biodiesel production process, is available at the global market. Some species of the genera  Streptomyces  have the ability to assimilate glycerol and convert it into valuable metabolic products. In the present study, the ability of  Streptomyces hygroscopicus  to assimilate waste glycerol and convert it into metabolic compounds with antifungal activity against four phytopathogenic fungi obtained from apple fruit samples expressing rot symptoms, was investigated. Production of antifungal metabolites by  S. hygroscopicus  was carried out in 3 l stirred tank bioreactor through 7 days. Fermentation was carried out at 27 °C with aeration rate of 1.5 vvm and agitation rate of 100 r.p.m. The aim of this work was to analyse bioprocess parameters and to determine at which stage of bioprocess the production of antifungal metabolites occurs. Activity of the cultivation liquid on two isolates of  Alternaria alternata  and two isolates of  Fusarium avenaceum  were determined every 12 h using in vitro well diffusion method. It was found that the maximum production of antifungal metabolites occurred at 108 hour of cultivation. Formed inhibition zones have shown that the produced antifungal metabolites have high efficacy on tested phytopathogenic fungi (inhibition zone diameter higher than 35 mm for all test organisms).

Production of plant protection agents in medium containing waste glycerol by Streptomyces hygroscopicus: Bioprocess analysis