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In this study, two bacilli strains, namely TN51 and TN69, previously isolated from Thua Nao, a Thai traditional fermented soybean, were studied in terms of their phenotypic and biochemical properties. Initially, both strains were subjected to morphological determination and a series of biochemical tests. Both were Gram-positive, endosporeforming bacilli. Based on 16S rRNA gene sequence analysis, the identities of strains TN51 and TN69 were confirmed as Bacillus subtilis and B. cereus, respectively. In addition, these two strains were also assessed for their antibiogram profiles. It was found that both strains were susceptible to chloramphenicol, erythromycin, kanamycin, tetracycline, and vancomycin and resistant to ampicillin and intermediately susceptible to bacitracin.\u

Characterization of Bacillus species exhibiting strong proteolytic activity isolated from Thua Nao