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Eight mycoparasitic Tichoderma harzianum isolates (TR01-TR05; TR07; TR09; TR10) were tested in vitro to examine their mycelial growth rate. Those were isolated from grapevine trunk disease (GTD) asymptomatic grapevine trunks from the Tokaj wine region, Hungary. The average linear growth rate (ALG) of the isolates was tested at five different temperatures (18.5-37°C). The mycoparasitic ability of the Trichoderma isolates against Fusarium oxysporum isolated from GTD symptomatic plants was also determined. 5 mm mycelial disks from the margin of seven days old colonies were cut, and put in 3 cm distance on PDA plates, than incubated at room temperature for 6 days. Finally growth inhibition value was calculated to express the mycoparasitic potential.

acta microbiologica et immunologica hungarica

Abstracts of the Annual Meeting of the Hungarian Society for Microbiology and EU FP-7 PROMISE Regional Meeting, October 15–17, 2014, Keszthely, Hungary