English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Malignant catarrhal fever (MCF) is a fatal disease syndrome responsible for mortality in domestic and wild ruminant species. MCF is caused by gammaherpesviruses – the ovine herpesvirus type 2 causing sheep-associated malignant catarrhal fever (SA-MCF) and alcelaphine herpesvirus type 1 known as wildebeest-associated MCF (WA-MCF). The present study described the cultural peculiarities of MCF virus isolated from different samples originated from dead and alive wild animals from the Sofia Zoo. MDBK, EBTR, RK, MA-104 and VERO cell cultures were used for the isolation of MCF virus. The best growth of viruses was observed on MDBK cell cultures. The CPE was characterised with forming of the syncytium and destruction of the monolayers 2–3 days after the virus adaptation. The CPE was different for obtained isolates. The isolates from gaur formed a bigger cell syncytium than that in the cell cultures infected with buffy coat from bisons where a cell syncytium of smaller size and shape was observed. The virus identification was performed by biochemical methods and PCR.

Isolation and identification of malignant catarrhal fever virus in cell cultures