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Information regarding metabolic profile in different physiological sates of dairy cows can assist veterinarians to monitor the herd health and productive performance. Furthermore, the relationships among the metabolic parameters can be used to detect the effect of each parameter on another one. The aim of the current research was to clarify the interactions among metabolic parameters in different physiological states of high producing Holstein dairy cows. The present study was carried out on 25 multiparous Holstein dairy cows divided into 5 equal groups: early, mid and late lactation; far-off and close-up dry. Blood samples were collected from all cows through jugular venipuncture and sera were separated to evaluate glucose, insulin, β-hydroxybutyric acid (BHBA), non-esterified fatty acids (NEFA), triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein and very lowdensity lipoprotein. There were negative and significant correlations between insulin and glucose in all studied groups (P 0.05). The correlations among studied metabolic parameters showed that providing the energy demands can prevent the ketogenic and lipolytic metabolisms in high producing dairy cows. Furthermore, information regarding the correlations among circulating metabolic parameters can be used to estimate the changing patterns of each metabolic parameter via evaluating another one.

Relationships among circulating metabolic biomarkers in healthy high-producing holstein dairy cows in different physiological states