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Molecular characterization using rep-PCR of native isolates of Bacillus thuringiensis, obtained from soil samples. Bacillus thuringiensis is a Gram-positive bacteria forming spores, which produces parasporal crystals of a proteic nature, toxic against various orders of insects and biodegradable and harmless to other species. This research use the experimental model, allowing, through observation techniques, microbiological and biochemical identification of B. thuringiensis from soil samples from municipalities of Cucuta, El Zulia, Los Patios, San Cayetano and Villa del Rosario, Norte de Santander, Colombia, and its subsequent characterization with molecular markers Bc-Rep and MB1. Microbiological and biochemical tests identified 10 isolates as B. thuringiensis; the results of phylogenetic analysis showed significant differences in the clusters obtained with Bc-Rep and MB1 markers. With Bc-Rep a low index of similarity (18%) was recorded, while with the marker MB1 a higher similarity index, 58%, was obtained. This work indicated a great genetic variability among isolates, showing that markers Bc-Rep and MB1 RESUMEN Bacillus thuringiensis es una bacteria Gram-positiva formadora de esporas, que produce cristales parasporales de naturaleza proteica, toxicos contra diferentes ordenes de insectos y biodegradables e inocuos para otras especies. Esta investigacion empleo el modelo experimental, que mediante tecnicas de observacion permitio, la identificacion microbiologica y bioquimica de B. thuringiensis a partir de muestras de suelo de los municipios de Cucuta, El Zulia, Los Patios, San Cayetano y Villa del Rosario, Norte de Santander, Colombia, y su posterior caracterizacion con los marcadores moleculares Bc-Rep y MB1. Se identificaron microbiologica y bioquimicamente 10 aislados como B. thuringiensis; los resultados del analisis filogenetico mostraron diferencias significativas en los agrupamientos obtenidos con los marcadores Bc-Rep y MB1. Con Bc-Rep se registro un indice de similaridad bajo (18%), mientras que con el marcador MB1 se obtuvo un indice mayor de similitud, 58%. En este trabajo se evidencio una gran variabilidad genetica entre los aislados, que mostraron a los marcadores Bc-Rep y MB1 como altamente efectivos para diferenciar cepas estrechamente

Caracterización molecular mediante rep-PCR de aislados nativos de Bacillus thuringiensis, obtenidos de muestras de suelo