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Presence of Colletotrichum acutatum and Colletotrichum gloeosporioides on leatherleaf fern, key lime, papaya, star fruit and mango in Costa Rica and Florida (United States). Anthracnose is a disease caused by several species of the genus Colletotrichum on different hosts. C. acutatum and C. gloeosporioides are 2 morphologically similar species that can cause similar disease symptoms on the same host. A total of 220 isolates of Colletotrichum species were collected from typical anthracnose lesions on leatherleaf fern (Rumohra adiantiformis), Key lime (Citrus aurantifolia), starfruit (Averrhoa carambola), papaya (Carica papaya) and mango (Mangifera indica) in Costa Rica and Florida. Isolates were characterized by colony morphology, benomyl sensitivity and PCR with specific primers for C. acutatum and C. gloeosporioides CaInt2 and Cg/fInt1, respectively. Of the 85 isolates obtained from mango, all 33 isolates from Florida were identified as C. acutatum, whereas all isolates from Costa Rica were C. gloeosporioides. Out of 60 isolates obtained from starfruit, 3 isolates from Costa Rica were RESUMEN La antracnosis es una enfermedad causada por varias especies del genero Colletotrichum en diferentes hospederos. C. acutatum y C. gloeosporioides son especies morfologicamente similares, que pueden causar sintomas similares en el mismo hospedero. Se colectaron 220 aislamientos de Colletotrichum de muestras con sintomas tipicos de antracnosis en helecho hoja de cuero (Rumohra adiantiformis), limon criollo (Citrus aurantifolia), carambola (Averrhoa carambola), papaya (Carica papaya) y mango (Mangifera indica) en Costa Rica y Florida, Estados Unidos. Los aislamientos se caracterizaron por morfologia de colonia, sensibilidad al benomil, y PCR con los iniciadores especificos para C. acutatum y C. gloeosporioides CaInt2 y Cg/fInt1, respectivamente; tambien se analizo la morfologia de las colonias de ambas especies. De los 85 aislamientos obtenidos de mango, todos los aislamientos de Florida fueron identificados como C. acutatum, mientras que todos los aislamientos de Costa Rica fueron C. gloeosporioides. De los 60 aislamientos de carambola, 3 provenientes de Costa Rica fueron C. acutatum (primer informe) y el resto fueron C. gloeosporioides. Todos los

agronomia costarricense

Presencia de &lt;i&gt;Colletotrichum acutatum&lt;/i&gt; y &lt;i&gt;Colletotrichum gloeosporioides&lt;/i&gt; en helecho hoja de cuero, limón criollo, papaya, carambola y mango en Costa Rica y Florida (Estados Unidos)