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espanolLos cementos de ionomero de vidrio convencionales se utilizan como materiales de restauracion provisional para uso dental, los cuales presentan varias ventajas como la adhesion a la fase mineral de los dientes. Por otro lado, las propiedades biologicas de los ionomeros de vidrio muestran diversos enfoques y resultados. En este trabajo se estudio la respuesta biologica in vitro de fibroblastos gingivales humanos en contacto con cementos comerciales de ionomero de vidrio: Mirafil® e Ionglass® y con sus respectivos extractos segun la norma ISO 10993. Los extractos de los cementos en los que se cultivaron las celulas estaban en diferentes concentraciones: de 0.1% a 100%. La actividad metabolica celular se midio usando el reactivo Alamar Blue®. Los resultados mostraron un efecto significativo sobre la actividad metabolica celular correlacionada con la concentracion de iones liberados (Al³+ y Ca²+) para ambos ionomeros, asi como las variaciones de pH de los medios de cultivo. Ello podria explicar la influencia por los iones y el pH del cultivo celular en la actividad metabolica celular. EnglishConventional glass ionomer cements are used as dental provisional restorative materials, which present several advantages such as adhesion to the tooth mineral phase among others. On the other hand, the knowledge about biological property of glass ionomers shows various approaches and results. In this work, it was studied the in vitro biological response of human gingival fibroblasts in contact with commercial cements of glass ionomer: Mirafil® and Ionglass® and with their extracts, according to ISO 10993. The extracts of the cements, in which the cells were cultured, were adjusted at different concentrations ranging 0.1% to 100%. The cellular metabolic activity of gingival fibroblasts was measured using the Alamar Blue® reagent. The results showed a significant effect on the cellular metabolic activity correlated with the concentration of liberated ions (Al³+ and Ca²+) for both ionomers, as well as the pH variations of the culture media. This could mean that the cellular metabolic activity is substantially influenced by ions and pH of the cell culture.

Effect of Ions Released and pH of Two Glass Ionomer Cements in Human Gingival Fibroblasts