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Objective: This study evaluated the effects of alternative self-etch application modes on resin-dentin microtensile bond strength (μTBS) of three commercially available “no wait” concept universal adhesives. Materials and methods: In this study extracted impacted non-carious human third molars were used. The flat surfaces were prepared in mid-coronal dentin and prepared with a 600-grit SiC paper. The three universal adhesives that were used are as follows: Clearfil Universal Bond Quick (CUQ, Kuraray Noritake, Japan), G-Premio Bond (GPB, GC Corp, Japan), and a self-curing universal adhesive “Tokuyama Universal Bond” (TUB; Tokuyama Dental, Japan). The following three different application procedures were used for the dentin surfaces: the adhesives were applied and immediately subjected to air-dry; the adhesives were applied followed by a 10-second wait; or the adhesives were rubbed for 10 seconds. Then composite resin was applied to the dentin surface and light cured. After storage in 37°C distilled water for 24 h, all the bonded teeth were cut into 1mm2 sections using a low-speed diamond saw (Micracut 125 Low Speed Precision Cutter, Metkon, Bursa, Turkey) under running water (n=15). The sections were subjected to a tensile force at a crosshead speed of 1mm/min in a testing apparatus (Microtensile Tester, Bisco, IL, USA) and μTBS values were measured. Data were analyzed using the Kruskal-Wallis test and Mann-Whitney U test. Failure modes were analyzed under a stereomicroscope. Results: Prolonged application time significantly affected the μTBS (p<0.005). A significant increase of μTBS on active application was observed for CUQ ODOVTOS-International Journal of Dental Sciences Serin-Kalay & Zaim: Effect of Alternative Self-Etch Applications on Dentin Bond Strength of “No Wait Concept” Universal Adhesives ODOVTOS-Int. J. Dent. Sc. | ISSN: 2215-3411. 283 and GPB. The TUB with an active application had a significantly lower μTBS value compared with the other adhesives. Conclusions: Prolonged application time caused significant improvement of bond strength in all adhesives. The active application is effective at increasing the dentin bond strength except for TUB.