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espanolIntroduccion. El cancer del mango ha ocasionado perdidas economicas en areas productivas en Costa Rica desde mediados de los 80. Los agentes causales han sido caracterizados solamente por metodos fenotipicos como Erwinia mangifera y E. herbicola. Objetivo. Confirmar, utilizando un enfoque molecular y fenotipico las especies de enterobacterias causantes del cancer bacterial del mango en Costa Rica. Materiales y metodos. Se tomaron frutas, ramas y troncos con sintomas en diferentes huertos de la provincia de Alajuela. Se realizo un aislamiento bacteriano y se evaluo la patogenicidad mediante la inoculacion de frutos y troncos de la variedad Tommy Atkins Los aislamientos positivos para la prueba de patogenicidad fueron re-inoculados, aislados e identificados para cumplir con los postulados de Koch. El aislado positivo CIBCM-Mg-115 que causo los sintomas fue analizado mediante la caracterizacion bioquimica completa y la identificacion molecular mediante analisis filogeneticos del ARNr 16S y los genes de mantenimiento atpD, gyrB, infB y rpoB. Resultados. De acuerdo con los datos obtenidos a partir de los analisis bioquimicos y moleculares se identifico a la cepa CIBCM-Mg-115 como Erwinia billingiae. Conclusion. E. billingiae corresponde a uno de los agentes causales del cancer bacteriano del mango (M. indica) en Costa Rica. EnglishIntroduction. In Costa Rica, bacterial canker of mango has caused economic losses in most of the productive areas since the mid-1980s. The causal agents have been identified only by phenotypic methods such as Erwinia mangifera and E. herbicola. Objective. To confirm, using a molecular and phenotypic approach, the species of the Enterobacteriaceae the cause bacterial canker of mango in Costa Rica. Material and methods. Fruits, branches, and trunks with symptoms were collected in different orchards in the Alajuela province. Bacterial isolation was performed, and pathogenicity was evaluated by inoculating fruits and trunks of the Tommy Atkins variety. The positive isolates for the pathogenic test were re-inoculated, isolated, and identified in order to fulfill Koch’s postulates. The CIBCMMg-115 positive isolate that caused symptoms was analyzed by complete biochemical characterization and molecular identification by phylogenetic analyses of 16S rRNA and the atpD, gyrB, infB, and rpoB housekeeping genes. Results. According to the data obtained from the biochemical and molecular analysis, the CIBCM-Mg-115 strain was identified as Erwinia billingiae. Conclusion. E. billingiae corresponds to one of the causal agents of bacterial canker on mango (M. indica) trees in Costa Rica.

Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica