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espanolIntroduccion. El genero Meloidogyne contiene muchas especies y el diagnostico correcto de estas debe basarse en el uso de multiples herramientas, mismas que en laboratorios de diagnostico permiten una evaluacion correcta y confiable, especialmente cuando podria tratarse de nematodos cuarentenarios. La identificacion adecuada de un fitonematodo es importante no solo para establecer una apropiada estrategia de control, sino que tambien para evitar su diseminacion a otras areas donde podria provocar danos que se traducirian en perdidas economicas. Objetivo. Realizar estudios taxonomicos y moleculares para confirmar la especie de Meloidogyne, asociada a una plantacion de cafe en la localidad de San Pedro de Barva, Heredia. Materiales y metodos. Se recolectaron muestras de suelo y raiz en una plantacion de cafe ubicada en Barva, Heredia, Costa Rica, las cuales se procesaron en el laboratorio de Nematologia de la Escuela de Ciencias Agrarias de la Universidad Nacional (UNA), por el metodo centrifugacionflotacion en solucion azucarada y tecnicas moleculares. Resultados. En promedio se contabilizaron 30 J2 en 100 cc-1 en suelo y 1000 J2 en 10 g-1 de raices del nematodo agallador. Tanto las tecnicas taxonomicas como moleculares permitieron identificar que las hembras y juveniles extraidos de raices de cafe correspondieron a Meloidogyne exigua. El analisis de las secuencias generadas mediante PCR y el uso de la enzima Dral confirmaron la identidad de la especie con un 99 % de similitud al compararla con secuencias de Costa Rica y Nicaragua, reportadas en el Genbank. Conclusion. Mediante el uso de diferentes tecnicas morfometricas y moleculares se identifico a M. exigua asociada a una plantacion de cafe. EnglishIntroduction. The genus Meloidogyne contains many species and their correct diagnosis of these must be based on the use of multiple tools, which in diagnostic laboratories allow a correct and reliable evaluation, specially for quarantine nematodes. The proper identification of a phytonematode is important not only to establish an appropriate control strategy, but also to avoid its spread to other areas where it could cause damage that would result in economic losses. Objective. To perform taxonomic and molecular studies to confirm the Meloidogyne species, associated to a coffee plantation located in Barva, Heredia. Materials and methods. Soil and root samples were collected in a coffee plantation located in Barva, Heredia, Costa Rica, which were processed in the Nematology laboratory of the School of Agricultural Sciences at the Universidad Nacional (UNA), using the centrifugation-flotation method in sugary solution and molecular techniques. Results. On average, 30 J2 100 cc-1 were counted in soil and 1000 J2 10 g-1 of roots of the root-knot nematode. Taxonomic and molecular techniques allowed to identify that the females and juveniles extracted from coffee roots corresponded to Meloidogyne exigua. Analysis of the sequences generated by PCR and the use of the DraI enzyme confirmed the identity of the species with 99 % similarity when compared to sequences from Costa Rica and Nicaragua, reported in the Genbank. Conclusion. Using different morphometric and molecular techniques, M. exigua associated with a coffee plantation was identified.

Identificación morfológica, morfométrica y molecular de Meloidogyne exigua (Göeldi 1887) en café (Coffea arabica)