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In vitro activity of nitrogen fixating and phosphate solubilizing bacteria. The aim of this work was to evaluate the in vitro nitrogen-fixing activity and phosphate solubilizing activities of endophytic bacteria isolated from colosoana grass (Bothriochloa pertusa). The grass tissue was collected from root, stems and leaves from different agroecological zones in the municipality of Corozal, Sucre, Colombia. Each tissue underwent surface cleaning, maceration, and isolation of endophytic bacteria in R 2 A agar medium. Population density was determined by plate counts as CFU/g of tissue, morphotypes were then identified by shape, color, size, and appearance. Each morphotype was evaluated in vitro for nitrogen-fixation and phosphate solubilization in specific culture media. All results were analyzed using the statistical program R. The test results showed a significant differences in terms of population density of endophytic bacteria per type of tissue. A significant differences were found between population density with respect to tissue; the highest values were observed in roots (1.61 x 10

Actividad in vitro de bacterias endófitas fijadoras de nitrógeno y solubilizadoras de fosfatos.