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The present study was conducted with an aim of evaluating the effects of sodium-heparin (20 I.U/ml) and di-potassium EDTA (1.0mg/ml) on the haematological parameters and blood cell morphology of freshwater fish Schizothorax labiatus. For blood sampling, fifteen fishes of uniform weight (250 ± 18.5 g) and length (25 ± 2.1 cm) were selected. All the haematological analysis were carried out immediately after sampling. An increase in the haematocrit level was observed in the samples collected with K 2 EDTA. Red blood cell counts were lower in K 2 EDTA collected samples as compared to heparinized samples. The WBC counts and haemoglobin concentrations did not differ significantly (p>0.05). Mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) increased in K 2 EDTA sample, while a significant decrease (p2EDTA as compared to heparinized sample. There was however, no significant difference (p>0.05) observed in WBC and thrombocyte area of K 2 EDTA and heparinized sample. The present study suggests the use of Na-heparin as a preferred anticoagulant for routine haematological analysis of fish blood.

journal of ecophysiology and occupational health/journal of ecophysiology and occupational health

Effects of Sodium-Heparin and Dipotassium EDTA on the Haematological Parameters and Blood Cell Morphology of Freshwater Fish &lt;I&gt;Schizothorax labiatus&lt;/I&gt; (Mcclelland, 1842)