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The artificial induction of polyploidy is a plant breeding technique used in plants of medicinal interest. However, few species of the genus Aloe have been subjected to this treatment. The objective of this research is to standardize the induction technique of polyploidy in vitroplants of Aloe vera (L.). An experimental design was performed with two groups (control and experimental), to which a cytogenetic study was applied pre and post treatment for three consecutive generations. Three concentrations of colchicine (0.05, 0.10 and 0.15%) and two exposure times (48 and 72 hours) were considered. The control vitroplants kept in distilled water (without colchicine) for 48 and 72 hours (T1 and T2 respectively) and those treated with colchicine ́s solution at 0.05% for 48 and 72 hours (T3 and T4 respectively), presented few cytogenetic changes, being diploid most of their cells. Plants treated with 0.10% colchicine ́s solution for 48 and 72 hours (T5 and T6 respectively) achieved chromosomal duplication in more than the 50% of the tissue. Those treated with a concentration of 0.15% for 48 hours (T7) showed chimeric tissue with a high predominance of

Induction of polyploidy with colchicine in vitroplants of Aloe vera (L.)