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Titulo corto: Primera incursion en la obtencion de curieles SPF en Cuba Titulo en ingles:  First foray into the production of specific pathogen-free guinea pigs in Cuba Resumen:  Resultados confiables y economicos solo son obtenidos cuando los animales de experimentacion son aislados de factores ambientales y biologicos, implantandose en el biomodelo una microbiota normal, lejos de la presencia de microorganismos patogenos. El objetivo de la investigacion fue obtener curieles libre de patogenos especificos por cesarea aseptica, mantenidos en aisladores y alimentados con dietas esteriles. Se realizaron 26 histerectomias. Los animales fueron alimentados con una formula modificada (L-477) en forma de papilla hasta los 21 dias y permanentemente despues la C-484 solida y granulada, esterilizadas a 121 o C/20 minutos o a 1,5Mrad. Ademas fueron suplementados con vitamina C y B1. El forraje o heno fue consumido a partir de la primera generacion. La microbiota gastrointestinal se administro por via oral en 0,5 ml de una dilucion de 10 -6 /g de contenido de la porcion final del ileon, ciego y principio del colon de curieles, a las 24 y 48 horas del nacimiento. Se utilizo para el monitoreo microbiologico caldo Tioglicolato, caldo Triptona Soya y caldo Saboraud incubados aerobicamente a temperatura de 55, 37y 25 o C respectivamente. Se obtuvieron 51 neonatos. La mortalidad mas alta se registro entre los primeros 10 dias de edad (58,8%). Se lograron 12 animales (3 machos y 9 hembras), 6 de las hembras se reprodujeron aproximadamente a los 9 meses de edad, lograndose 11 crias por parto normal. La metodologia aplicada permitio obtener curieles libres de Salmonella sp, Pasteurella sp, Streptococcus del tipo A y C, Bordetella bronchiseptica , Toxoplasma gondii , Virus Sendai y parasitos internos y externos. Palabras clave : curieles, libre de patogenos especificos, aisladores, histerectomia y dietas esteriles. Abstract:  Reliable and economic results are only obtained when the animals are isolated of environmental and biological factors, that could interfere the course of the investigation and when it is being performed in them, a normal microflora balanced far from the presence of pathogen microorganisms. 26 histerectomies were carried out. The animals were fed with a modified formula of L-477 in the form of porridge until the 21 st day of age and, and after then with the C-484 solid and granulated, sterilized at 121 o C/20 minutes or 1,5Mrad. The forage or hay was consumed by the first generation since the 6 months of age. The gastrointestinal flora was orally administered with 0,5 ml of a dilution of 10-6/g of the contents of the ileum final portion, cecum and first part of the colon of conventionalized Dunkin Hartley guinea pigs, 24 and 48 hours after the birth. It was used for microbiological bacteria monitoring Thioglycolate broth, Tryptic Soy broth and Sabouraud broth incubated aerobically at temperatures of 55, 37 and 25 o C respectively. 51 newborns were obtained. The highest mortality was registered during the first 10 days of age (58,8%). 12 animals were born: 9 female and 3 males. 6 of the female reproduced approximately at the age of 9 months and having 11 normal delivery suckling . The guinea pigs were free the following specific pathogens: Salmonella sp., Pasteurella sp., Streptococcus of the group A y C. Bordetella bronchiseptica , Toxoplasma gondii , Sendai Virus, beside internal and external parasites. Key words: guinea pigs, specific pathogen free, isolators, hysterectomy and sterile diets.

Primera incursión en la obtención de curieles libre de patógenos específicos en Cuba