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AbstrakPenyakit busuk pangkal batang pada kelapa sawit (Elaeis guineensis Jacq.) disebabkan oleh Ganoderma boninense Pat. Bakteri selulolitik dari rizosfer kelapa sawit dapat dijadikan agen hayati dalam menghambat pertumbuhan G. boninense. Penelitian ini bertujuan untuk mendapatkan bakteri selulolitik dari rizosfer kelapa sawit dan menentukan efektivitasnya dalam menghambat pertumbuhan G. boninense. Tahap penelitian meliputi pengambilan sampel dari rizosfer kelapa sawit, isolasi, pemurnian, uji aktivitas selulolitik, dan uji daya hambat terhadap G. boninense serta identifikasi isolat bakteri yang potensial. Indeks selulolitik bakteri ditentukan dengan pewarnaan congo red 0,1%. Aktivitas penghambatan dilakukan dengan menentukan persentase daya hambat bakteri dalam menghambat G. boninense. Uji efektivitas dengan membandingkan hasil uji T pada taraf 5%. Hasil menunjukkan bahwa dari rizosfer kelapa sawit diperoleh 19 isolat bakteri selulolitik dengan indeks selulolitik tertinggi 4,38 pada isolat LBS1. Berdasarkan uji T dari efektivitas bakteri selulolitik terhadap G. boninense menunjukkan 6 isolat bakteri (LBS3, LBS4, DBS1, DBS7, SBS2 dan SBS6) memiliki nilai efektif atau berpotensi sebagai antagonis dengan persentase daya hambat tertinggi sebesar 40,17% isolat DBS1 yang merupakan genus Flavobacterium.  Harapan kedepannya, bakteri selulolitik yang diperoleh dapat dijadikan agen biokontrol terhadap G. boninense.Abstract Basal stem root disease in oil palm tree (Elaeis guineensis Jacq.) is caused by Ganoderma boninense Pat. infection. Cellulolytic bacteria from rhizosphere can be used as agents to inhibit G. boninense growth as pathogenic fungi. Purpose of the research is to obtain cellulolytic bacteria from oil palm tree rhizosphere and to determine their effectiveness in inhibiting G. boninense growth. The research stages included sampling from the oil palm tree rhizosphere, isolation, purification, cellulolytic activity test and dual culture test against G. boninense and identification of effective isolates. The cellulolytic index of bacteria was determined by congo red 0.1%. Antagonistic test was conducted to determine persentation of isolate bacteria in inhibit G. boninense and effectiveness test by comparing result of T test at the level 5%. The results showed that there are 19 isolates of cellulolytic bacteria from oil palm tree rhizosphere with highest cellulolytic index 4.38 (isolates LBS1). Based on T test of the effectiveness cellulolytic bacteria against G. boninense showed 6 isolates (LBS3, LBS4, DBS1, DBS7, SBS2, and SBS6) had effective values or potential antagonists with highest percentage inhibition 40.17% isolate DBS1 genus of Flavobacterium. Hope that in the future, cellulolytic bacteria obtained can be used as biocontrol agents for G. boninense.

AKTIVITAS ANTAGONISTIK BAKTERI SELULOLITIK ASAL RHIZOSFER KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP Ganoderma boninense Pat.