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Basidiomycetes cultures were screened for the ability to actively express the cellulases complex. Nutrient media with various forms of sugars were used. From 22 cultures of macromycetes (14 species), a group of six cultures with high level activities of extracellular (Il-11 I. lacteus – 70 IU, Fvv F. velutipes – 78 IU, Pe-1 P. eryngii – 87 IU, Ps-1 L. sulphureus – 83 IU, Mg M. giganteus – 74 IU) and intracellular (Sc-51 S. commune – 102 IU) cellulase complex was selected. Cultures of the species exhibit notable differences in the expression of enzymes, which indicates a significant influence of genetic factors on the process of producer selection. Endo-1,4-β-D-glucanases isozymes for most fungi had a molecular weight of 55 kDa and above, except for S. commune, which had more variability of conformation and weight 12–55 kDa. The culture of Il-11 I. lacteus on media with Avicel and filter paper had the highest activity, its endo- and exo-1,4-β-D-glucanases activities ranged 37–39 IU/mL and 18–20 IU/ mL, respectively. The culture of S. commune Sc-51 is able to accumulate a significant amount of intracellular cellulases, but the production of culture fluid with high viscosity complicates technological manipulations and increases processing time. The obtained data allowed us to isolate an Il-11 I. lacteus culture with stable expression and high activity of the cellulases complex at different carbon sources.

Cellulases of basidiomycetes for the development of cellulose bioconversion technologies