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In recent decades aquariculture has emerged as a globally growing million dollar industry comprising cultivation of various freshwater and marine species of finfish as well as shellfish.1 Expansion of the ornamental fish culture industry has enabled Indian producers to market locally and internationally. One of the key drivers for rapid popularity of the hobby of ornamental fish keeping over the past 50 years is the development of manufactured or formulated feed.2 Correct formulation of feed is the key component for improving nutrient digestibility as well as supplementing the metabolic needs, thereby reducing the maintenance cost and also combat water pollution. For normal growth and survival of ornamental fish the lipids are important sources of energy and fatty acids.3 Improved growth performance was found in swordtail with increase in dietary lipid from 8% to 16% with same protein level. Additionally the source of lipid in the ovary depends on the muscle lipid content which is a vital indicator of the reproductive performance. To support cell membrane flexibility at lower water temperature fatty acids with low melting points are required.2 Dietary levels of dietary docosahexaenoic acid significantly affected egg hatchability in the freshwater common carp, Cyprinus carpio4 one of the food fishes having significant commercial value.

Improvised protocol for quantitative determination of crude fat in fish feeds