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Triglycerides (TG) can be generated by esterification process of three hydroxyl (-OH) groups of glycerol with three molecules of fatty acids. Triglyceride acts not only in human metabolisms but also as energy sources and dietary fat transporter. The determinations of triglyceride levels in urine, blood and food is important for clinical or medical analysis to determine the clinical disorders due to abnormal levels of triglyceride. Standard methods for triglyceride determination in serum based on fluorometric, chromatographic, and high performance liquid chromatographic (HPLC) methods are not extensively available and unsuitable for point-of-care applications due to expensive instrumentation and complicated sample pre-processing. Therefore, biosensors offer advantages for triglyceride determination due to relatively low cost, ease of use and good selectivity. The most recent reports of triglyceride biosensors found in literature1–3 are based on interactions of three enzymes, lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO). Pundir et al.4 reported an electrochemical sensor for triglyceride by measuring the oxygen consumption or the amount of hydrogen peroxide produced by the enzymatic reactions.

international journal of  biosensors and bioelectronics

The Electroanalytical Detection of Triglyceride Concentrations in Olive Oil using Modified Screen Printed Electrodes with Ionic Liquid [1-(2-Ethoxyethyl)-1-Methylpyrrolidinium Bis(Trifluoromethylsulfonyl)Imide]-Lipase