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A method for simultaneous determination of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP) and creatine phosphate (PCr) by high-performance liquid chromatography is described. This method was applied to the isolated intestinal smooth muscle tissue of guinea pig taenia caeci weighing approximately 30 mg. It was found that one g of the muscle tissue contained 3.55 mumol PCr, 2.40 mumol ATP and 0.477 mumol ADP.

nihon heikatsukin gakkai zasshi

Method for simultaneous determination of creatine phosphate and adenine nucleotides in the intestinal smooth muscle of guinea-pig taenia caeci using high-performance liquid chromatography.