English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Molecular markers make it possible to monitor the directional introgression of alien genetic material to the genome of wheat. Among the diverse molecular markers, the PLUG (PCR-based landmark unique gene) markers have the greatest advantage for analyzing distant hybrids due to the ability to amplify polymorphism fragments on closely related species and to detect simultaneously three genomes within one homoeological group in one PCR. A collection of 41 entries of different breeding stages developed in Russian State Agrarian University as a result of crossing of bread wheat (Triticum aestivum L.) and wheat T. timopheevii Zhuk. was studied. All hybrids were resistant to leaf rust and powedery mildew natural infection. Using this collection of distant hybrids, we demonstrated for the first time the possibilities of PLUG markers for identifying alien introgressions in the genome of bread wheat from T. timopheevii. In order to increase the degree of polymorphism between the entries, we additionally processed the products of amplification of PLUG markers with highfrequency restriction endonucleases TaqI and HaeIII. The entries were found to carry both single and multiple introgressions. The latter entries with introgressions in more than one homeological group are of particular interest. The heterogeneity of not only the late generations of entries, but also the entries from the nursery of the preliminary cultivar estimation, which may be associated with secondary chasmogamy, is shown. As a result of the studies, 14 PLUG markers for the long and short chromosome arm of each homeological group have been selected, which can be recommended for the detection of the genetic material of T. timopheevii in distant hybrids of T. timopheevii 1⁄2 T. aestivum. The advantages of PLUG markers as a tool for monitoring the transfer of alien hereditary material into the genome of bread wheat are discussed.

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING