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The Oomycete Phytophthora infestans (Mont.) de Bary, the causal agent of the disease known as late blight, is primarily responsible for the decreased in production performance and potato crops worldwide. The integration of the complete R genes sequences in the potato genome using Agro-transformation appears an alternative to be considered in the fight against this pathogen. The Rpi-blb2 gene (R gene) from the wild species Solanum bulbocastanum Dunal shows a broad resistance to isolates of P. infestans, making it an important candidate for plant breeding studies. This paper reports the integration of the Rpi-blb2 gene into potato var. Desiree genome by Agrobacterium tumefaciens - mediated transformation system, the molecular characterization of 29 events transformed and whole plant infection with isolate POX67 of P. infestans from Peru. Desiree events [Rpi-blb2] 4 and Desiree [Rpi-blb2] 30, showed a substantial resistance to P. infestans infection confirming complete transfer of the Rpi-blb2 gene from a wild species to a cultivated species by genetic transformation.

Agrotransformación y evaluación de la resistencia a Phytophthora infestansen Solanum tuberosum L. variedad Désirée