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Tuberculosis (TB) is an infectious disease primarily of humans which produces high morbidity and mortality. Dogs are also susceptible to Mycobacterium tuberculosis infections; therefore, the present study searched for evidence of the presence of this pathogen in 58 dogs that live with human pulmonary TB patients in Lima, Peru. Chest Xray was performed and pharyngeal swab, saliva, bronchial fluid, urine and feces samples were taken from dogs. Fluorescence microscopy was performed with auramine O staining and TB culture by the Lowenstein Jensen and the Microscopic Observation Drug Susceptibility (MODS) methods. Epidemiological data was collected to define the patient