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Twenty percent of the Swiss albino mice administered with a single dose (60 mg/kg) of oxazepam dissolved in dimethyl sulphoxide (DMSO) died within 7 days. Similarly high mortality rate (37%) was observed in diazepam sensitive mice derived from a Swiss albino stock. In contrast, zero mortality was observed in Swiss albino mice administered with DMSO or diazepam (35 mg/kg). Mortality at 60 mg/kg diazepam was only 10%. The high mortality caused by oxazepam in Swiss albino mice seemed to be strain-related as only 7% mortality was observed with identically treated BALB/c mice. Since DMSO is the only convenient vehicle for the administration of oxazepam by injection, it is suggested that a suitable strain must be selected for experimentation in order to avoid unnecessary loss of animals.

Mortality in Swiss Albino Mice after i. p. Injection of Oxazepam in Dimethyl Sulphoxide