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Lignocellulosic biomass is a relatively inexpensive and abundant feedstock for biofuel production. The key to unlocking the recalcitrance of lignocelluloses is an effective pretreatment process. A promising new pretreatment method for lignocellulosic biomass is the use of ionic liquids (ILs). In this study, wood flour was partially dissolved in the novel ionic liquid 1-butyl-3-methylimidazolium acesulfamate ([BMIM]Ace) mixed with different organic solvents (1,4-dioxane, acetone, methanol, DMSO, and DMF) followed by precipitation in water. Hemicelluloses were successfully extracted from the carbohydrate-enriched residues by an alkaline ethanol solvent. Sugar analysis of the hemicellulosic fractions indicated that xylose (63.25-74.85%) was the major sugar component, while small amounts of glucose (4.85-14.40%) and galactose (4.49-7.32%) were also observed. Molecular weights of these fractions varied between 49.330 and 60.760 g/mol as determined by GPC. NMR studies revealed that the hemicelluloses had a backbone of β-(1→4)-linked-D-xylopyranosyl units and were branched mainly through 4-O-methyl-α-D-glucuronic acid. The thermal degradation behavior of the hemicellulosic fractions showed that the most significant degradation occurred between 242 and 300 °C.

Characterization of Hemicelluloses Obtained from Partially Delignified Eucalyptus Using Ionic Liquid Pretreatment