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Laccase activity and stability were improved and modified through the interaction between laccase amino acid residues (e.g., amino groups (-NH2) and carboxyl groups (-COOH)) and the chemical reagents thiourea dioxide (TDO) and L-phenylalanine methyl ester hydrochloride (L-PME). Compared with the control sample, the activity of the laccase modified with these chemical reagents was increased by 209% and 50%, respectively. The stability of the laccase modified with L-PME increased by 56.9%. However, the laccase modified with TDO only improved slightly. It was clear that reagent L-PME was more efficacious than TDO. The paper formed with addition of L-PME-modified laccase exhibited better performance in terms of tensile strength, bursting strength, and tear strength, which increased by 13%, 10%, 9%, respectively, compared with the paper formed with unmodified laccase.

Chemical Modification of Laccase from Aspergillus oryzae and its Application in OCC Pulp