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Novel bioflocculants (BS-MBF) were prepared using the wastewater supernatant from anaerobic co-digestion of corn straw and molasses wastewater as a nutrient resource. Acetic acid and ethanol were the dominant fermentation products during the anaerobic digestion process and were estimated to be 50.5% and 30.0%, respectively, after 150 d of operation. Equal volumes of bioflocculant producing bacteria F2 (Rhizobium radiobacter) and F6 (Bacillus sphaericus) were mixed to form F+, which was inoculated to wastewater supernatant at different times. A maximum flocculation activity of 91.3% was achieved, and 2.32 g/L of purified bioflocculant was extracted when a compound medium from 110-d wastewater supernatant was used. The removal efficiencies of heavy metals from simulated electroplating wastewater were tested by using these prepared bioflocculants. The optimal conditions for heavy metal removal to BS-MBF were found to be at 374 mg/L at an initial pH of 6.0 and a contact time of 40 min. The adsorption capacities for Cu2+ and Zn2+ reached more than 90%, while for Cr6+ it reached approximately 30%. Overall, the study showed for the first time that wastewater supernatant from anaerobic co-digestion of corn straw and molasses wastewater can be used for producing bioflocculants, which can be effectively used to remove heavy metals from electroplating wastewater.

Production of Bioflocculants Prepared from Wastewater Supernatant of Anaerobic Co-Digestion of Corn Straw and Molasses Wastewater Treatment