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The selection of dairy cattle is dictated by improvement of yield and composition of milk. Genotyping tests give breeders the opportunity to use previously unavailable information to increase the frequency of beneficial alleles in their herds. A QTL influencing milk yield and composition is located in the centromeric end of chromosome 14. The polymorphism being analyzed is a K232A substitution (lysine to alanine amino acid change) in exon VII of the diacylglycerol acyl transferase 1 (DGAT1) gene. DGAT1 is a key acyltranferase in triglyceride biosynthesis. DGAT1 encodes an enzyme which catalyzes the final step of mammalian triglyceride synthesis from carbohydrates. The K232A substitution in the DGAT1 gene has an effect on milk production traits by having a higher milk fat content. The two nucleotide polymorphisms in exon VII (aa  gc) give rise to an MwoI restriction fragment length polymorphism (RFLP) which is used to identify the three DGAT1 genotypes (AA, AG, GG). Research has shown that the genotype AA of the DGAT1 gene is associated with higher milk fat. Since the United States has been selecting for milk volume and protein, herds in the US should have a higher proportion of the G allele. The objective of this study was to examine the association of DGAT1 genotypes with fat content, lipid profiles, citrate levels, and MFGM proteins in the Cal Poly Herd. Our results

Polymorphic Variation of the DGAT1 Gene in Cal Poly Holstein and Jersey Breeds and the Correlation of Milk Fat Content, and Genetic Values