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O presente trabalho teve como objetivo isolar e identificar bacterias e fungos presentes na microbiota intestinal de ovinos higidos e acometidos por diarreia. Foram coletadas amostras de fezes de 50 animais higidos e 16 animais com diarreia. A colheita foi realizada por introducao de swab no reto do animal. O material foi colocado em tubos contendo o meio de transporte Stuart, e em seguida cultivado em meios seletivos para Staphylococcus, Salmonella/Shigella, Listeria, Bacillus cereus, Clostridium, Pseudomonas ; para o isolamento de fungos, foi empregado o meio agar sabouraud dextrose. As bacterias gram-negativas isoladas foram identificadas pelo sistema API 20 E., enquanto que as bacterias Gram-positivas foram submetidas aos testes:  coagulase, catalase, NaCl 5%, oxidase, novobiocina e DNAse. As leveduras foram semeadas em CHROM agar e posteriormente identificadas pela formacao do tubo germinativo, provas da urease e fermentacao de carboidratos. Os agentes isolados das amostras provenientes de diarreia foram avaliados quanto a susceptibilidade a agentes antimicrobianos pelo metodo de difusao em placa, em agar Muller Hinton. Observou-se que a microbiota normal de ovinos estava constituida por bacterias gram-positivas: Staphylococcus aureus, S. intermedius, Staphylococcus coagulase negativo , Lactobacillus, Bacillus cereus, Clostridium perfringens, Listeria spp e Listeria monocytogenes ; Gram-negativas: Escherichia coli (enteropatogenica) , Salmonella spp e Pseudomonas aeruginosa; e por fungos da especie Candid as albicans . Nos animais acometidos por diarreia verificou-se que o agente etiologico foi a bacteria Escherichia coli enteropatogenica. A cepa de E. coli isolada apresentou uma ampla resistencia aos antibioticos testados, dentre eles tetraciclina, ampicilina, norfloxacilina, sulfazotrim, eritromicina,  oxacilina, penicilina,  ciprofloxacilina, acido nalidixico e cefalotina. Concluiu-se que a microbiota intestinal de ovinos possui grande proporcao de bacterias, algumas patogenicas, que nao apresentaram efeitos deleterios devido a condicao sanitaria higida do animal. Tambem ficou evidente a importância da identificacao do agente causador da diarreia, e em caso de diarreia bacteriana, a realizacao de antibiograma, para que a eleicao do antibiotico seja feita de maneira correta e com isso, o tratamento eficaz. SUMMARY This study isolates and identifies bacteria and fungi present in the intestinal tract of healthy and diarrhea suffering sheep. We collected stool samples from 50 healthy animals and 16 animals with diarrhea. Harvesting was performed by introducing swab in the animal rectum. This material was placed into tubes containing Stuart transport medium, then cultured in media selective for Staphylococcus , Salmonella / Shigella , Listeria , Bacillus cereus , Clostridium , Pseudomonas , fungi were isolated using the Sabouraud dextrose agar medium. The gram-negative bacteria isolated were identified by the API 20 E. system, whereas Gram-positive bacteria were subjected to the tests: coagulase, catalase, NaCl 5%, oxidase, novobiocin and DNAse. Yeasts were plated on CHROM agar and subsequently identified by germ tube formation, urease tests and fermentation of carbohydrates. Agents isolated from samples of diarrhea suffering sheep were evaluated for susceptibility to antimicrobial agents by plate diffusion method on Muller Hinton agar. It was observed that healthy sheep flora was constituted by gram-positive bacteria: Staphylococcus aureus , S. intermedius , coagulase-negative Staphylococcus , Lactobacillus , Bacillus cereus , Clostridium perfringens , Listeria spp. and Listeria monocytogenes ; as well as the Gram-negative bacteria: Escherichia coli (enteropathogenic), Salmonella spp. and Pseudomonas aeruginosa , and fungal species Candidas albicans . In animals suffering from diarrhea, it was found that the causative agent was the enteropathogenic Escherichia coli . The strain of E. coli isolated showed a wide resistance to the tested antibiotics, including tetracycline, ampicillin, norfloxacilin, sulfazotrim, erythromycin, oxacillin, penicillin, ciprofloxacilin, nalidixic acid and cephalothin. It was concluded that sheep intestinal flora has a large amount of bacteria, some pathogenic that have no deleterious effects when the animal is healthy. It was also evident the importance of identifying the causative agent of diarrhea, and in case of bacterial diarrhea, performing an antibiogram in order to select the best antibiotic to treat the condition in the most effective way.

MICROBIOTA INTESTINAL DE OVINOS HÍGIDOS E COM DIARREIA / INTESTINAL MICROBIOTA OF HEALTHY AND DIARRHEA SUFFERING SHEEP