English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Fibroblast growth factor 2 (FGF2), a member of FGF family, binds with FGF receptors (FGFR) to initiate biological functions in various somatic cells. However, little is known regarding the role of FGF2/FGFR on oocyte meiosis. In this study, we investigated expression patterns and functions of FGF2/FGFR during  in vitro  maturation (IVM) of mouse cumulus-oocyte complexes (COCs). Among four FGFRs,  Ffgr1  was the most abundant in COCs. The transcripts for  Fgf2  and  Ffgr1  in COCs increased during IVM.  Ffgr1  was present in oocytes and cumulus cells, while  Fgf2  was present in only cumulus cells. Treatment of COCs with the selective FGFR inhibitor SU5402 blocked oocyte meiotic progression and downregulated expression of  Bmp15  and  Gdf9 . In contrast, supplement of FGF2 promoted oocyte meiotic progression and upregulated  Bmp15  and  Gdf9  expression. Inhibition of FGFR with SU5402 reduced cumulus expansion and expressions of  Ptx3 ,  Has2  and  Tnfaip6 . Treatment with FGF2 increased  Ptx3  and  Has2  expression. Inhibition of FGFR had no effect on meiotic progression of denuded oocytes (DOs). However, co-culture of DOs with COCs or supplementation with FGF2 promoted meiotic progression of DOs. Inhibition of FGF2/FGFR signaling also downregulated  Ffgr1  expression, while supplemental FGF2 upregulated  Fgfr1  expression. Furthermore, inhibition of FGFR in COCs interrupted the c-Mos/MAPK pathway and maturation-promoting factor (MPF), as indicated by downregulation of oocyte  c-mos  and  Ccnb1  transcripts, respectively. Overall, this study suggests that FGF2 produced by cumulus cells, activates a FGF2/FGFR autocrine/paracrine loop within COCs to regulate cumulus expansion and oocyte meiosis. These findings reveal a novel role for FGF2/FGFR signaling during  in vitro  maturation of COCs.

FGF2/FGFR signaling promotes cumulus–oocyte complex maturation in vitro