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A study was conducted to determine the effects of pregnancy-specific protein B (PSPB) and prostaglandin F2 alpha (PGF2 alpha) on bovine luteal cell progesterone, prostaglandin E2 (PGE2) and oxytocin production in vitro. Corpora lutea were enucleated from multiparous cows with normal oestrous cycles during the mid-luteal (days 10-12; n = 5) or late-luteal (days 17-18; n = 5) stage. Mixed large and small cells (1.5 x 10(5) cells per well) were incubated in 500 microliters modified Ham's F-12 medium. Cells were incubated for 18 h before treatments were added. Cells were treated with PSPB (0, 2.5, 5.0 micrograms) and PGF2 alpha (0, 100, 200 ng) in a 3 x 3 factorial arrangement. After treatments were added, media samples were collected at 6 and 12 h. During the 18 h pretreatment incubation, progesterone, PGE2 and oxytocin production was similar between the prospective treatment groups. Progesterone production was greater (P &lt; 0.001) by mid-stage than by late-stage cells. In addition, progesterone decreased (P &lt; 0.001) as incubation time increased. Progesterone production was not affected by PGF2 alpha, but PSPB increased (P &lt; 0.02) progesterone at the 5.0 micrograms dose. Late-stage luteal cells produced more (P &lt; 0.001) PGE2 than did mid-stage cells; PGE2 production decreased (P &lt; 0.001) with increased incubation time. Luteal PGE2 production increased in response to PSPB treatment (P &lt; 0.01) and PGF2 alpha treatment (P &lt; 0.001). Luteal oxytocin production was greater (P &lt; 0.01) by mid-stage compared with late-stage cells. Oxytocin production decreased (P &lt; 0.001) with incubation time in mid-stage cells, but in late-stage cells oxytocin production was similar over time. Neither PSPB nor PGF2 alpha had an effect on oxytocin. These results indicate that PSPB does not affect luteal oxytocin, but does increase progesterone and PGE2 production. In addition, PGF2 alpha increases luteal PGE2, but does not affect progesterone or oxytocin production. These data do not show an interaction between PSPB and PGF2 alpha in regulating bovine luteal cell endocrine function.

reproduction

Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2 