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Eight experiments were performed to validate an extraction technique for canine acrosin and to quantitate the acrosin activity of fresh and frozen-thawed spermatozoa. Acrosin activity from fresh spermatozoa differed amongst dogs and was influenced by the interval since previous ejaculation. Freezing and thawing spermatozoa induced a loss of acrosin activity that differed with the extender in which the spermatozoa were frozen. The assay of acrosin activity, in conjunction with motility estimates, provides a more complete evaluation of the efficacy of seminal extenders in attenuating freezing injury than do motility estimates alone.

Acrosin activity of canine spermatozoa as an index of cellular damage