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We have re-examined whether immunity to spermatozoa can be detected by spleen cell migration assays in inseminated female mice. Unlike previous results, however, sperm suspensions inhibited the migration of splenic leucocytes obtained from mated as well as control mice. Small differences were observed between leucocytes from untreated virgin mice and leucocytes from mice immunized with spermatozoa in adjuvant. In these experiments lymph node cells draining the injection site were added to the spleen cells.

Failure of spleen cell migration assays to detect cell-mediated immunity to spermatozoa after natural mating in female mice