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Summary. HeLa and BHK-21 cells, which are not related immunologically to fowl cells, were cultured and the effects of these cells on the survival and fertilizing ability of fowl spermatozoa were investigated. When spermatozoa were incubated at 41\s=deg\C with these cells, sperm motility was maintained for 5\p=n-\6days. Fertilizing ability was also retained after incubation at 41\s=deg\Cfor 2 days. Cultured cells also contributed to the maintenance of motility of bull and ram, but not boar, spermatozoa.

Effects of HeLa and BHK-21 cells on the survival of fowl, bull, ram and boar spermatozoa in vitro