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A modification of the leucocyte adherence-inhibition test was developed to study cell-mediated immunity to spermatozoa. The test is highly reproducible and requires very low numbers of leucocytes. Deposition of spermatozoa in the genital tract of the female mouse by one normal mating is sufficient to sensitize the female to sperm antigens and the degree of sensitization is independent of the number of matings and the strain of male, and is not enhanced by subsequent pregnancy.

Detection of cell-mediated immunity to spermatozoa in mice and man by the leucocyte adherence-inhibition test