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Although the fetal fluids of sheep have been examined in detail in conscious catheterized animals after about 60 days of pregnancy (Mellor & Slater, 1971, 1972, 1973a; Mellor & Matheson, 1977) there is no comparable information for goats. We have therefore examined the composition of the fetal fluids from conscious goats for comparison with the sheep data. Twenty-five pregnant British Toggenburg mixed breed goats (40-80 kg) at known stages of pregnancy were used ; 15 carried twins, 9 had single fetuses and 1 triplets. The animals were penned individually and received 0-8-1-0 kg pelleted feed (Ruminant A: Seafield Mill, Midlothian) and 0-30-5 kg hay per day. They were handled daily, including sampling from a jugular vein, for 4 weeks and were fasted for 48 h before operation. Between 81 and 111 days ofgestation catheters were inserted into the allantoic and/or amniotic sacs of 36 fetuses (Mellor, 1970a) and into the bladders of 3 of them (Mellor, Williams & Matheson, 1972). Fetal fluids, fetal urine and maternal plasma were sampled daily (Mellor et ai, 1972) before feeding at 09.30-10.30 h, until term or abortion. Eleven goats (Group A) received no progesterone, 10 (Group B) were given 20 mg progesterone (Organon Laboratories Ltd., Surrey) i.m. in oil once daily from the day before until 3-5 days after operation, the dose then being decreased progressively to zero during the next 2-4 days, and 4 goats (Group C) were given ¬ 5 mg progesterone/day from the day of operation until term. All analytical procedures were as described by Mellor & Slater (1973b), apart from those for fructose (Roe, 1934) and calcium and magnesium (Mellor & Matheson, 1977). Progesterone concentrations were measured using a modi¬ fied competitive protein-binding assay based on those described by Thorburn, Bassett & Smith (1969) and Challis, Heap & Illingworth (1971). The extraction effciency was 89 ± 1-3% (S.E.M.) (n = 14), the values for distilled water blanks were always <0-2 ng/ml, and the interassay coefficient ofvariation

Changes in fetal fluid composition during the last 60 days of gestation in goats