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It has been reported that there is an increase in the activity of acid phosphatase, alkaline phosphatase and lactic dehydrogenase in different regions of the rabbit oviduct within 14 hr after mating (Varma & Talwar, 1973; Varma et al., 1974). Concomitant with the increase in the total activity of the lactic dehydro- genase (LDH; l(+)-lactate: NAD+ oxidoreductase, EC 1.1.1.27), there is a shift in the isoenzymic pattern of the enzyme, and the M-form of the enzyme (which favours the formation oflactate) increases markedly in the organ (Varma et al., 1974). The stimulus for these changes is not yet known, but is presumably hormonal or neural. The present investigations were undertaken to examine the factor(s) responsible for these metabolic changes. Sexually mature female rabbits, bred in the Institute colony and weighing 2\p=n-\2\m=.\5 kg, were used. The rabbits were caged separately for at least 3 weeks before use and were allowed free access to food and water. Ovulation was induced by mating with a fertile buck, and confirmed at autopsy by the presence of ovulation points. The animals were killed by injecting air into the ear vein or with a lethal dose of sodium pentobarbital (Nembutal Nembuta Nembut Nembutal: Abbott Laboratories, Chicago, Illinois). The oviducts were rapidly removed, trimmed of supporting mesentery on an ice-cold glass \m=.\latm and flushed with 0-15 M-NaCl to remove spermatozoa. The infundibulum, ampulla and isthmus were separated by the procedure described by Greenwald (1961). The tissues were blotted, weighed to the nearest 0-1 mg, minced and homogenized in 3-0 ml of 100 mM-sodium phosphate buffer (pH 7-5). The 105,000 g supernatants were used as the enzyme source for the LDH assay. The activity of LDH was determined as described by Varma et al. (1974). A total volume of 1 ml assay medium contained 50 mM-phosphate buffer, 0-15 mM-NADH and 2 mM-pyruvate. For the H- and M-types of the enzyme, the assays were performed in the presence of 2 mM- (H and M activity) and 30 mM- (M activity) pyruvate concentrations. Protein concentrations were determined by the method of Lowry et al. (1951) with crystalline bovine serum albumin as the standard. The hormones used were: LH (NIH-LH-S18) at 20 /ig/kg body wt; prolactin (NIH-P-S10) at 20 /¿g/kg body wt; 20a-hydroxypregnen-3-one (Ikpharm,

NATURE OF THE STIMULUS OF THE COPULATION-INDUCED RISE IN LACTIC DEHYDROGENASE ACTIVITY IN THE RABBIT OVIDUCT